Histopathologic, Histometric and Immunohistochemical Features of Lesional and Non-lesional Skin of P
Journal of Pan-Arab League of Dermatologists
Vol. 19, No. 1, February 2008 Page 75-82
Histopathologic, Histometric and Immunohistochemical Features of Lesional and Non-lesional Skin of Patients with Atopic Dermatitis
Sameh K Attia, Hesham M Ahmad, Wael H Abdel-Razek, Sahar S Boray Department of Dermatology, Faculty of Medicine, Al-Minya University, Al-Minya, Egypt Key words: Atopic dermatitis, eosinophils, epidermal thickness, CD markers
Abstract
Although atopic dermatitis (AD) is one of the most common skin diseases, its pathogenesis is not completely understood. This work aims to study the histopathologic, histometric and immunohistochemical features of lesional and non-lesional skin of patients with AD. The study included 49 patients with AD: 3 acute, 15 subacute and 31 chronic cases. Skin biopsies were obtained from lesional and non-lesional skin of all patients and sections were utilized for hematoxylin and eosin, immunohistochemical staining for some CD markers and epidermal thickness measurements. The early lesions of AD showed perivascular lymphohistiocytic infiltrate (100%), epidermal spongiosis (50%) and acanthosis (38.9%), whereas late lesions showed acanthosis and perivascular lymphohistiocytic infiltrate (100%) and compact orthokeratosis (54.8%). Tissue eosinophilia was noticed in 17 (54.8%) cases with chronic and 3 (20%) cases with subacute AD lesions. Epidermal measurements increased from 155 μm ± 60 in early lesions to 200 μm ± 80 in late lesions (p=0.04). Lesional skin showed strong expression of CD68, moderate expression of CD3, weak expression of CD4 and CD8 but negative expression of CD20, whereas non-lesional skin showed moderate expression of CD3, CD8 and CD68 but only mild expression of CD4 and CD20. Our data suggest that the histopathologic, histometric and immunohistochemical changes of AD skin differ between early and late AD lesions and that apparently normal skin of AD patients is histologically abnormal.
Journal of Pan-Arab League of Dermatologists
Vol. 19, No. 1, February 2008 Page 75-82
Histopathologic, Histometric and Immunohistochemical Features of Lesional and Non-lesional Skin of Patients with Atopic Dermatitis
Sameh K Attia, Hesham M Ahmad, Wael H Abdel-Razek, Sahar S Boray Department of Dermatology, Faculty of Medicine, Al-Minya University, Al-Minya, Egypt Key words: Atopic dermatitis, eosinophils, epidermal thickness, CD markers
Abstract
Although atopic dermatitis (AD) is one of the most common skin diseases, its pathogenesis is not completely understood. This work aims to study the histopathologic, histometric and immunohistochemical features of lesional and non-lesional skin of patients with AD. The study included 49 patients with AD: 3 acute, 15 subacute and 31 chronic cases. Skin biopsies were obtained from lesional and non-lesional skin of all patients and sections were utilized for hematoxylin and eosin, immunohistochemical staining for some CD markers and epidermal thickness measurements. The early lesions of AD showed perivascular lymphohistiocytic infiltrate (100%), epidermal spongiosis (50%) and acanthosis (38.9%), whereas late lesions showed acanthosis and perivascular lymphohistiocytic infiltrate (100%) and compact orthokeratosis (54.8%). Tissue eosinophilia was noticed in 17 (54.8%) cases with chronic and 3 (20%) cases with subacute AD lesions. Epidermal measurements increased from 155 μm ± 60 in early lesions to 200 μm ± 80 in late lesions (p=0.04). Lesional skin showed strong expression of CD68, moderate expression of CD3, weak expression of CD4 and CD8 but negative expression of CD20, whereas non-lesional skin showed moderate expression of CD3, CD8 and CD68 but only mild expression of CD4 and CD20. Our data suggest that the histopathologic, histometric and immunohistochemical changes of AD skin differ between early and late AD lesions and that apparently normal skin of AD patients is histologically abnormal.