Some Oxidative Stress Parameters of Seminal Plasma and Computer Aided Sperm Analysis Motility Parame
Journal of Pan-Arab League of Dermatologists
Vol. 18, No. 2, June 2007 Page 15- 24
Some Oxidative Stress Parameters of Seminal Plasma and Computer Aided Sperm Analysis Motility Parameters in Infertile Males with Varicocele
Before and After Varicocelectomy
Yousry M Moustafa and Mohamed Abd-Alatif*
Departments of Dermatology & Andrology and Biochemistry*, Faculty of Medicine, Mansoura University, Egypt
Abstract
Background: Oxidative stress with elevated reactive oxygen species (ROS) and decreased levels of total antioxidant capacity (TAC) of seminal plasma may pay a role in the pathophysiology of infertility in patients with varicocele. Malondialdhyde is the degradation product of sperm membrane lipid peroxidation. Sperm protein
oxidation leads to loss of critical thiol groups which have antioxidant activity. Varicocelectomy was reported to reduce ROS levels and increase TAC of seminal plasma. Computer aided sperm analysis (CASA) motility parameters increases after varicocelectomy.
Aim: To evaluate the relationship between malondialdhyde, total thiol groups and total antioxidant capacity levels in seminal plasma and sperm motility characteristics in infertile males with varicocele by using CASA, and to evaluate the effect of varicocelectomy on these oxidative stress indices and CASA parameters.
Patients and Methods: seminal plasma levels of malondialdhyde, total thiol groups and TAC and CASA motility parameters were estimated for 35 infertile patients with varicocele before and 6 months after high ligature varicocelectomy and 20 fertile control.
Results: This study showed statistically significant increase in seminal plasma malondialdhye, significant decrease in total thiol groups, TAC and CASA motility parameters in infertile patients with varicocele than control and than the same group of patients 6 months after varicocelectomy while the difference between the patients after varicocelectomy and control were non significant except for lateral head displacement (ALH) which was found to be significantly lower in patients than control.
Conclusion: Varicocele leads to oxidative stress of sperms with elevation of sperm lipid peroxidation product malondialdhyde, protein oxidation with decease in seminal antioxidant total thiol groups and seminal TAC. Varicocele was also associated with decreased CASA motility parameters. Both abnormal oxidative stress and CASA motility parameters returned to nearly normal measures 6 months after varicocelectomy
Journal of Pan-Arab League of Dermatologists
Vol. 18, No. 2, June 2007 Page 15- 24
Some Oxidative Stress Parameters of Seminal Plasma and Computer Aided Sperm Analysis Motility Parameters in Infertile Males with Varicocele
Before and After Varicocelectomy
Yousry M Moustafa and Mohamed Abd-Alatif*
Departments of Dermatology & Andrology and Biochemistry*, Faculty of Medicine, Mansoura University, Egypt
Abstract
Background: Oxidative stress with elevated reactive oxygen species (ROS) and decreased levels of total antioxidant capacity (TAC) of seminal plasma may pay a role in the pathophysiology of infertility in patients with varicocele. Malondialdhyde is the degradation product of sperm membrane lipid peroxidation. Sperm protein
oxidation leads to loss of critical thiol groups which have antioxidant activity. Varicocelectomy was reported to reduce ROS levels and increase TAC of seminal plasma. Computer aided sperm analysis (CASA) motility parameters increases after varicocelectomy.
Aim: To evaluate the relationship between malondialdhyde, total thiol groups and total antioxidant capacity levels in seminal plasma and sperm motility characteristics in infertile males with varicocele by using CASA, and to evaluate the effect of varicocelectomy on these oxidative stress indices and CASA parameters.
Patients and Methods: seminal plasma levels of malondialdhyde, total thiol groups and TAC and CASA motility parameters were estimated for 35 infertile patients with varicocele before and 6 months after high ligature varicocelectomy and 20 fertile control.
Results: This study showed statistically significant increase in seminal plasma malondialdhye, significant decrease in total thiol groups, TAC and CASA motility parameters in infertile patients with varicocele than control and than the same group of patients 6 months after varicocelectomy while the difference between the patients after varicocelectomy and control were non significant except for lateral head displacement (ALH) which was found to be significantly lower in patients than control.
Conclusion: Varicocele leads to oxidative stress of sperms with elevation of sperm lipid peroxidation product malondialdhyde, protein oxidation with decease in seminal antioxidant total thiol groups and seminal TAC. Varicocele was also associated with decreased CASA motility parameters. Both abnormal oxidative stress and CASA motility parameters returned to nearly normal measures 6 months after varicocelectomy