Immunohistochemical and PCR Study of the Effect of Narrow-band UVB Treatment on Survivin Expression
Journal of Pan-Arab League of Dermatologists
Vol. 19, No. 2, June 2008 Page 53- 61
Immunohistochemical and PCR Study of the Effect of Narrow-band UVBTreatment on Survivin Expression in Patients with Chronic Plaque
Psoriasis
Dalia M Shaaban, Hassan T Sheir*, Naglaa F Ghoname**
Departments of Dermatology & Venereology, Pathology* and Microbiology** Tanta University
Abstract
Background: Psoriasis is an inflammatorypapulosquamous skin disease characterized byincreased keratinocyte proliferation. Survivin isa member of inhibitors of apoptosis protein thathas a role in the regulation of apoptosis andcell division. This study aimed at evaluation ofsurvivin expression both immunohistochemically
and by PCR in patients with chronicplaque psoriasis before and after treatment withnarrow band UVB in order to elucidate its rolein the pathogenesis of psoriasis.
Patients and Methods: The study included 15patients with chronic plaque psoriasis. Skin biopsieswere taken from uninvolved as well asfrom involved skin before and after treatmentwith narrow band UVB. Survivin expressionwas detected both immunohistochemically andby PCR.
Results: Survivin expression was statisticallysignificantly higher in involved skin comparedto uninvolved skin both immunohistochemicallyand at mRNA level.There was a statisticallysignificant reduction in survivin expression afternarrow band UVB treatment using bothmethods. Moreover, a statistically significantdirectcorrelation was detected between survivinexpression and PASI score both beforeand after treatment.
Conclusions: Survivin may play a putative rolein the pathogenesis of psoriasis. Downregulatorsof survivin that were previously used intreatment of cancer couldbe tried as a newtreatment option in psoriasis.
Journal of Pan-Arab League of Dermatologists
Vol. 19, No. 2, June 2008 Page 53- 61
Immunohistochemical and PCR Study of the Effect of Narrow-band UVBTreatment on Survivin Expression in Patients with Chronic Plaque
Psoriasis
Dalia M Shaaban, Hassan T Sheir*, Naglaa F Ghoname**
Departments of Dermatology & Venereology, Pathology* and Microbiology** Tanta University
Abstract
Background: Psoriasis is an inflammatorypapulosquamous skin disease characterized byincreased keratinocyte proliferation. Survivin isa member of inhibitors of apoptosis protein thathas a role in the regulation of apoptosis andcell division. This study aimed at evaluation ofsurvivin expression both immunohistochemically
and by PCR in patients with chronicplaque psoriasis before and after treatment withnarrow band UVB in order to elucidate its rolein the pathogenesis of psoriasis.
Patients and Methods: The study included 15patients with chronic plaque psoriasis. Skin biopsieswere taken from uninvolved as well asfrom involved skin before and after treatmentwith narrow band UVB. Survivin expressionwas detected both immunohistochemically andby PCR.
Results: Survivin expression was statisticallysignificantly higher in involved skin comparedto uninvolved skin both immunohistochemicallyand at mRNA level.There was a statisticallysignificant reduction in survivin expression afternarrow band UVB treatment using bothmethods. Moreover, a statistically significantdirectcorrelation was detected between survivinexpression and PASI score both beforeand after treatment.
Conclusions: Survivin may play a putative rolein the pathogenesis of psoriasis. Downregulatorsof survivin that were previously used intreatment of cancer couldbe tried as a newtreatment option in psoriasis.
