Expression of CD30 Variant and CD30 Ligand mRNA in the Skin and Blood of Atopic Dermatitis
Journal of Pan-Arab League of Dermatologists
Vol. 19, No. 1, February 2008 Page 83-89
Expression of CD30 Variant and CD30 Ligand mRNA in the Skin and Blood of Atopic Dermatitis
Zeinab Abd El-Samad and Amal El-Bendary Departments of Dermatology & Venereology and Clinical Pathology Faculty of Medicine, Tanta University
Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease in which a pathogenic role of Th2 cells has been supposed. Some studies have demonstrated a positive correlation between the expression of CD30 or soluble CD30 and the severity of allergic disease. However the role of CD30 is still a questioned in atopic dermatitis. The aim of this work was to evaluate the expression of CD30 variant (CD30 V) and CD30 ligand (CD30 L) m RNA in the skin lesion and on peripheral blood mononuclear cells (PBMCs) of atopic dermatitis patients and to detect the possible relation with total serum IgE and disease severity. This study included 20 moderate to severe AD patients and 10 healthy non-atopic control. Bloods samples were collected for estimation of total serum IgE and isolation of PBMCs. Skin punch biopsies were taken for DNA extraction and evaluation of CD30V/CD30L mRNA using RT-PCR. Positive CD30V / CD30L mRNA expression was found in 25% and 30% on PBMCs of AD patients and in 45% and 50% in skin lesion respectively, while the control showed negative expression. No significant correlation was found between CD30V/CD30L and total serum IgE, but positive correlation exists with the severity of the disease. It could be concluded that CD30 molecule is an important immuno-regulatory mechanism affecting pathogenesis of AD which may help in development of more effective strategies for prevention and treatment of AD.
Journal of Pan-Arab League of Dermatologists
Vol. 19, No. 1, February 2008 Page 83-89
Expression of CD30 Variant and CD30 Ligand mRNA in the Skin and Blood of Atopic Dermatitis
Zeinab Abd El-Samad and Amal El-Bendary Departments of Dermatology & Venereology and Clinical Pathology Faculty of Medicine, Tanta University
Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease in which a pathogenic role of Th2 cells has been supposed. Some studies have demonstrated a positive correlation between the expression of CD30 or soluble CD30 and the severity of allergic disease. However the role of CD30 is still a questioned in atopic dermatitis. The aim of this work was to evaluate the expression of CD30 variant (CD30 V) and CD30 ligand (CD30 L) m RNA in the skin lesion and on peripheral blood mononuclear cells (PBMCs) of atopic dermatitis patients and to detect the possible relation with total serum IgE and disease severity. This study included 20 moderate to severe AD patients and 10 healthy non-atopic control. Bloods samples were collected for estimation of total serum IgE and isolation of PBMCs. Skin punch biopsies were taken for DNA extraction and evaluation of CD30V/CD30L mRNA using RT-PCR. Positive CD30V / CD30L mRNA expression was found in 25% and 30% on PBMCs of AD patients and in 45% and 50% in skin lesion respectively, while the control showed negative expression. No significant correlation was found between CD30V/CD30L and total serum IgE, but positive correlation exists with the severity of the disease. It could be concluded that CD30 molecule is an important immuno-regulatory mechanism affecting pathogenesis of AD which may help in development of more effective strategies for prevention and treatment of AD.